Enzymatic-Crosslinked Hydrogels

Several enzymes (e.g., transglutaminases, Sortase A, peroxidase) are used by the biomaterials community to form hydrogels. Enzymatic crosslinking is both cell-friendly and orthogonal to other crosslinking modalities. In our laboratory, we often work with hyaluronan-transglutaminase (HA-TG), which has been used as a scaffold for chondrocytes, progenitor cells and neurons. The excellent biological properties of HA-TG can be retained when we have developed modifications of these materials for use in extrusion bioprinting, two-photon patterning, and casting.