Multiphoton-Biofabrication

Over the past 20 years, light-mediated biofabrication has seen increasingly widespread use in the field of tissue engineering. Among light-based techniques multiphoton-biofabrication (MPB) also often referred to as two photon stereolitography offers complementary opportunities to other techniques such as volumetric printing due to its sub micrometer resolution. MPB relies on the near simultaneous absorption of multiple photons which only happens in a tiny volume of the focal point due to its low probability. In our group we use MPL for various aspects including tissue on chip and fabrication of architecture hydrogels. In particular we look to improve ablation of components found in the body to ensure more representative extracellular matrix-cell interactions. In addition, we use MPB to 4D print hydrogels to create shape changing scaffolds and we use MFB to fabricate high precision moulds for curvature assays and microfluidic chip fabrication.